골다공증 유도 백서경골에 티타늄 임플란트 매식 시 저출력 초음파 적용이 골 치유에 미치는 영향
Effect of low intensity pulsed ultrasound (LIPUS) on bone healing around a titanium implant in the tibia of osteoporosis-induced rats
황영섭, 전현준, 신상훈, 정인교, 김규천, 김철훈, 김욱규,
소속 상세정보
황영섭 ( Hwang Young-Sup ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
전현준 ( Jeon Hyun-Jun ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
신상훈 ( Shin Sang-Hun ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
정인교 ( Jung In-Kyo ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강해부학교실
김철훈 ( Kim Cheol-Hun ) - 동아대학교의료원 치과학교실 구강악안면외과
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620110370050386
Abstract
Introduction:Osteoporosis is a major health problem in the elderly that involves changes in the properties of bone as well as impaired bone healing around a titanium implant in both humans and animals. This study examined effect of low intensity pulsed ultrasound (LIPUS) on the bone healing process around a titanium implant in osteoporosis-induced rats.
Materials and Methods : Sixteen rats were divided into two groups. A control group with osteoporosis induced by removing both ovaries and an experimental group of rats that were applied with LIPUS after osteoporosis had been induced. A screw type titanium implant (diameter, 2.0 mm: length, 3.5 mm, Cowell-Medi, KOREA) was placed into the tibias of 16 rats. The control and experimental group contained 8 rats each. The rats were sacrificed at 1, 2, 4, and 8 weeks after implantation to examine the histopathology and immunochemistry.
Results:The histopathology examination revealed earlier new bone formation in the experimental group than the control group. In particular, at 1 week after implantation, more new bone matrix and collagen were observed around the implant of the experimental group compared to the control group. Immunochemistry analysis showed that the level of OPG expression of the experimental group was higher in the early stages than in the control group. After 8 weeks, the levels of OPG expression were similar in both groups. The expression level of receptor activator of nuclear factor kB ligand (RANKL) was stronger in the experimental group than the control group. After 4 weeks, the level of RANKL expression was similar in both groups.
Conclusion:These results suggest that the application of LIPUS to implantation can promote bone healing around titanium in osteoporosis animals.
키워드
Osteoporosis; Dental implant; Low intensity pulsed ultrasound
원문 및 링크아웃 정보
등재저널 정보